Investigate the effect of particles settling out and interacting with the bottom of the microscope slide. Our samples are from Bangs Labs.  Functionalized silica spheres.   [[https://wiki.uchicago.edu/download/attachments/154371469/PDS%20702%20Web.pdf?version=1&modificationDate=1547566659000&api=v2|Example data sheet.]] [[https://wiki.uchicago.edu/download/attachments/154371469/TechNote%20206%20Web.pdf?version=1&modificationDate=1547566682000&api=v2|Bangs Labs Technote.]] For our spheres the maximum settling velocity in water at room temperature should go as Vm=5.448x10−5(ρs−1)d2Vm=5.448x10−5(ρs−1)d2 {FIXME $V_{m} = 5.448x10^{-5} (\rho_{s} - 1) d^{2}$ . VmVm {FIXME $V_{m}$  = maximum settling velocity in (cm/sec). ρsρs {FIXME $\rho_{s}$  = sphere density (g/cm3). d2d2 {FIXME $d^{2}$  = mean diameter of sphere (μm). Data for our microspheres.  Assume a drop depth of 0.01" (0.0254cm). | Particle Diameter (μm) | VmVm {FIXME $V_{m}$ (cm/s) | Settling Time (s) | Settling Time (m) | | 0.57 | 1.77e-5 | 1435 | 23.9 | | 0.96 | 5.02e-5 | 506 | 8.4 | | 1.54 | 1.29e-4 | 196.9 | 3.3 | | 3.17 | 5.47e-4 | 46.4 | 0.77 | | 4.89 | 1.30e-3 | 19.5 | 0.33 | | 8.37 | 3.82e-3 | 6.7 | 0.11 |